Culture of rat mesenchymal stem cells on PHBV-PCL scaffolds: analysis of conditioned culture medium by FT-Raman spectroscopy

Abstract Mesenchymal stem cells (MSCs) have great potential for application in cell therapy and tissue engineering procedures because of their plasticity capacity to differentiate into different types. Given the widespread use MSCs, it is necessary better understand some properties related osteogenic differentiation, particularly those linked biomaterials used engineering. The aim this study was develop an analysis method using FT-Raman spectroscopy identification quantification biochemical components present conditioned culture media derived from MSCs with or without induction differentiation. All experiments were performed between passages 3 5. For analysis, cultured on scaffolds composed bioresorbable poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) poly(ε-caprolactone) (PCL) polymers. (GIBCO®) inoculated onto pure polymers 75:25 PHBV/PCL blend (dense porous samples). plate itself as control. maintained DMEM (with low glucose) containing GlutaMAX® 10% FBS at 37oC 5% CO2 21 days. collected analyzed probe functional groups, well possible molecular variations associated differentiation metabolism. permitted identify groups specific molecules medium such cholesterol, phosphatidylinositol, triglycerides, beta-subunit polypeptides, amide regions hydrogen bonds proteins, addition DNA expression. In study, exhibited limited resolution since can express similar even same stretching vibrations, a fact that makes difficult. There no readings samples studied. conclusion, did not meet expectations under conditions